Click on configuration tab and select the laser icon b. The owneroperator and user of this product are responsible for proper. Introduction the ibif leica confocal microscope is made available on a feeforusehour basis to all users who have been trained. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. Leica tcs sp ii comes with a context sensitive online help system that teaches you the different functions of the system. Only with the leica tcs sp2 scanhead is userswitchable between upright and inverted microscopes and alignment. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. Leica tcssp2 upright confocal laserscanning microscope. Drawingalineontheannotationsheet240 drawingarectangleontheannotationsheet240. Ts16 user manual all instructions required in order to operate the product to a basic level are contained in the user manual. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens.
So its always useful to have an instruction manual available, just in case one needs to look up some camera or handling detail. The buttons provided on the user interface of the leica confocal software. You will discover useful features that you breezed over the first time. Select machine for the configuration and dm6000 for the microscope. This manual is the first section of a three part leica tcs sp5 user guide. Leicas mseries of rangefinder cameras are marvels of mechanical engineering and provide the photographer with rich and sometimes complex functionality. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system. Laser lines at 488nm and 543nm for excitation of cy2 and cy3 were provided by an ar laser and a hene laser. The newest technology is implemented in the leica tcs spe and this system is the only confocal in its class providing true spectral detection. Leica tcs sp2 laser scanning spectral confocal microscope part.
The leica tcs spes streamlined approach makes it accessible to every user, beginners and experts alike. The leica tcs sp2 upright confocal laserscanning microscope is located in room 170 and can accommodate specimens labeled with nearly all stains except those requiring ultraviolet excitation. We described here a protocol for sc isolation from green fluorescent protein gfpexpressing mice and their transplantation into murine muscles. Superior image performance, all the configurable as a personal, multiuser or advanced imaging center multiple innovations from the pacesetter in confocal microscopy prepared for the future. Leica sp8 tcs users manual v2 university of oklahoma. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. Leica ts16 user manual optical survey equipment ltd. Manual total station for standard measurement tasks. Pcmicroscope scanner power laser power laser key las af ok 1. Read carefully through the user manual before you switch on the product. First look at a hugely complicated bit of optics kit.
Legal notes leica microsystems heidelberg gmbh page 3 operating instructions leica tcs sp2 english art. User manual for leica tcs sp2 systems 1 table of contents. Typing your password identifies you as a valid user for this system. Refer to 1 safety directions for further information. The laser light emitted from the leica tcs sp2 used in accordance with. Leica tcs sp2 upright confocal laserscanning microscope. Abstract satellite cell sc transplantation represents a powerful strategy to investigate sc biology during muscle regeneration. Double click on the las x software found on the desktop. Refer to 6 safety directions for further information. Click ok to start the initialization of the las af leica application suite for advanced fluorescence 11. User guide to the ibif leica tcs sp8 mp confocal microscope.
Select machine for the configuration and dmi8 for the microscope. Please contact your sales person with possible substitutions. Table of contents leica microsystems heidelberg gmbh operating instructions leica tcs sp2 englisch art. Leica tcs sp2 rs, laser scanning spectral confocal. Leica tcs sp2 laser scanning spectral confocal microscope. Leica tcs sp2 laser scanning spectral confocal microscope part 1. Leica tcs sp2 rs, laser scanning spectral confocal microscope parts. This manual is the first section of a three part leica tcs sp5 user guide edited by donald pottle. If you are a new user, you should start with the chapter running the leica tcs software, use the software for a while, and then read through the rest of the book. Database contains 1 leica dm lm manuals available for free online viewing or downloading in pdf. The leica tcssp2 upright confocal laserscanning microscope is located in room 170 and can accommodate specimens labeled with nearly all stains except those requiring ultraviolet excitation. Superior image performance, all the configurable as a personal, multiuser or advanced imaging center. Leica sp2 experiments are saved as multiple tiff files in a single folder.
This can contain many different series acquired during the one experiment. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. Leicatcs the confocal microscope system for highest demands in multiuser facilities. Leica tcssp5 upright confocal laserscanning microscope. They either trigger actions directly or open dialog windows. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Mar 07, 2016 first look at a hugely complicated bit of optics kit. Click ok to start the initialization of the las x leica application suite for advanced fluorescence 11. Leica dm lm manuals and user guides, microscope manuals. For sale leica tcs sp2 rs, laser scanning spectral confocal microscope unit is pull from system and i super condition.
Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. Name descriptionformat captivate technical reference manual overall comprehensive guide to the product and apps. This book is both a tutorial and a reference manual for the leica tcs. Images were collected with a leica tcs sp2 aobs confocal microscope mannheim, germany with 25x and 60x oil immersion optics. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Risk assessment form for chemistry cardiff university. Leica tcs sp2 confocal microscope zmbh imaging facility short descripon. Inverted leica dm irb, ire 2 precision focusing nosepiece dm rxe. Leica tcs sp5 confocal laser scanning microscope user guide photometric 5 model upright dm 6000s.
Leica tcs sp5 upright confocal laserscanning microscope. With the las x control software, leica microsystems offers an interface, which shows all you need to know about your confocal on one screen. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Make sure that your sample slides are clean and sealed. White light laser system 470 to 670 nm tunable in steps of 1 nm. The new leica flexline ts07 is a manual total station for mid to highaccuracy survey and stakeout tasks.
We intend this book for the novice and the experienced user. User manuals, guides and specifications for your leica dm lm microscope. The tcs spe is very easy to use and first results are quickly achieved, even by confocal newcomers. This manual contains important safety directions as well as instructions for setting up the product and operating it. Most advanced manual total station for highend tasks. If you have any suggestions or improvements for this user manual, please contact the leica branch office in your country.
The system provides the full range of scan speeds at the highest resolution. Enter you name, the date, the time, and the account number in the user log book. Provides an overview of the product together with technical data and safety directions. Notes on device safety and installation requirements 2a. Manual leica software tcs sp2 free download as pdf file.
It is recommended setting up a separate user id for each user set up by the system administrator. Manual x, y and z stage control is via the knobs attached to the microscope controller, or by the manual xy stage control on the stage itself. Manual leica tcs sp ii university of massachusetts. Confocal microscopy facility schepens eye research. Leica tcs sp2 is a laser scanning confocal microscope with electronically controlled and freely. The user can select the appropriate option for his or. Double click on the las x icon to start the software. Further safety information can be found in the safety guidance notes for each system kept in lab e0. User guide to the ibif leica tcs sp8 mp confocal microscope this version. Leica tcs sp2 first confocal with excitation from uv to ir hc optics system ensures superlative optical performance first use of aotf for laser modulation in a confocal first dedicated objectives for confocal scanning cs with improved axial color specification working with the user, for the user ernst leitz i, 18431920. It has a number of hardware features, specifically the objectives, the stage, and the. Purchase congratulations on the purchase of a leica ts11ts15. Turn on the fluorescent lamp top left of the computer monitor.
Use windex and cotton balls or kimwipes to clean coverslips. Leicamicrosystemsheidelberggmbh general leicatcssp2usermanualenglish partno159330053vers31102002 page5of307 general howtousethismanual aboutthisbook. Container contents a gkl211 battery charger b gfz3 diagonal eyepiece c gev189 data cable usbrs232 d. Leica tcs sp2 confocal microscopes products home leica leica microsystems. The buttons provided on the user interface of the leica confocal. Along with many tiffs, the folder also contains a text description in the. It is recommended setting up a separate user id for each. Buttons are marked with icons andor have an often abbreviated english label. Training manual leica tcs sp5 confocal microscope september 2010. To retrieve the contents of the online help system. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. If playback doesnt begin shortly, try restarting your device. Manual leica software tcssp2 angular resolution web.
Log into user profile press ctrl, alt and delete simultaneously enter userid and password 5. To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Manual for leica sp2 confocal microscope enter you name, the date, the time, and the account number in the user log book. Leica tcs sp5 confocal laser scanning microscope user guide. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical.
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